PubMed: 11-Nor-9-Carboxy-Δ8-Tetrahydrocannabinol, 7-Carboxy Cannabidiol, and 11-Nor-9-Carboxy-Δ9-Tetrahydrocannabinol in Urine by LC-MS/MS

PubMed: 11-Nor-9-Carboxy-Δ8-Tetrahydrocannabinol, 7-Carboxy Cannabidiol, and 11-Nor-9-Carboxy-Δ9-Tetrahydrocannabinol in Urine by LC-MS/MS

Methods Mol Biol. 2024;2737:161-174. doi: 10.1007/978-1-0716-3541-4_16.

ABSTRACT

Δ8-Tetrahydrocannabinol (Δ8-THC) and cannabidiol (CBD) are increasingly popular cannabinoids. Measuring metabolites in urine is an important tool for detecting use and/or exposure as well as for monitoring elimination of these two drugs. Distinguishing between the metabolite 11-nor-9-carboxy-Δ8-tetrahydrocannabinol (Δ8-THC-COOH) and the analogous metabolite of the more common and naturally abundant Δ9-THC: 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (Δ9-THC-COOH) is analytically challenging due to structural similarities between the two compounds. Here, we present a method for separating the positional isomers Δ8-THC-COOH and Δ9-THC-COOH as well as 7-carboxy cannabidiol (CBD-COOH) in urine that includes reverse-phase solid-phase extraction (SPE), followed by liquid chromatographic separation with a perfluorophenyl column, and detection by tandem mass spectrometry (LC-MS/MS).

PMID:38036820 | DOI:10.1007/978-1-0716-3541-4_16

https://pubmed.ncbi.nlm.nih.gov/38036820/?utm_source=Chrome&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1Ds1JEbG0OWaBdqM3tTUGjkFhFGaOtMecPdpuvzbuubWi6d9Fn&fc=20231022105433&ff=20231201012156&v=2.17.9.post6+86293ac November 30, 2023 11:00 am