PubMed: 3M™ Molecular Detection Assay 2-Salmonella for Detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: Targeted Matrix Extension AOAC® Performance Tested MethodsSM 091501

PubMed: 3M™ Molecular Detection Assay 2-Salmonella for Detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: Targeted Matrix Extension AOAC® Performance Tested MethodsSM 091501

J AOAC Int. 2022 Jun 23:qsac068. doi: 10.1093/jaoacint/qsac068. Online ahead of print.

ABSTRACT

BACKGROUND: The 3M™ Molecular Detection Assay 2-Salmonella method is based on real time loop-mediated isothermal amplification when used with the 3M Molecular Detection System for the rapid and specific detection of Salmonella in enriched products. The 3M Molecular Detection Assay 2 – Salmonella was approved as AOAC® Performance TestedSM Certificate No. 091501 and as AOAC Official Method of AnalysisSM 2016.01.

OBJECTIVE: This matrix extension study evaluated the 3M Molecular Detection Assay 2 – Salmonella for detection of Salmonella in dried cannabis flower [>0.3% delta 9-tetrahydrocannabinol (THC)] and dried hemp flower (≤0.3% THC) at a 10 g test portion size.

METHODS: Matrix studies in dried cannabis and hemp flowers followed procedures outlined in 3M Molecular Detection Assay 2 – Salmonella Product Instructions and Standard Method Performance Requirements (SMPRs®) for Detection of Salmonella species in Cannabis and Cannabis Products (AOAC SMPR 2020.002). The method was evaluated at low, high, and non-contaminated levels.

RESULTS: Results showed no statistically significant difference between the presumptive positive 3M Molecular Detection Assay 2 – Salmonella results and the SMPR 2020.002 recommended cultural confirmations.

CONCLUSION: This study demonstrates that the 3M Molecular Detection Assay 2 – Salmonella is a reliable method for the rapid and specific detection of Salmonella in dried cannabis flower and dried hemp flower.

HIGHLIGHT: The 3M Molecular Detection Assay 2 – Salmonella method is suitable for the rapid and specific detection of Salmonella in dried cannabis flower and dried hemp flower.

PMID:35737412 | DOI:10.1093/jaoacint/qsac068

#CBD #Hemp https://pubmed.ncbi.nlm.nih.gov/35737412/?utm_source=Chrome&utm_medium=rss&utm_campaign=None&utm_content=1jYCQzi_o_qLYr-oQfnMhShgOXkvGma3vcnBGJtrBhuJMOvEVJ&fc=None&ff=20220624070017&v=2.17.6 June 23, 2022 10:00 am

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