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PubMed: Acyclic Diterpene Phytol from Hemp Seed Oil (<em>Cannabis sativa</em> L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-Macrophages

PubMed: Acyclic Diterpene Phytol from Hemp Seed Oil (<em>Cannabis sativa</em> L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-Macrophages

Foods. 2022 Aug 7;11(15):2366. doi: 10.3390/foods11152366.

ABSTRACT

Seeds from non-drug varieties of hemp (Cannabis sativa L.) have been used for traditional medicine, food, and fiber production. Our study shows that phytol obtained from hemp seed oil (HSO) exerts anti-inflammatory activity in human monocyte-macrophages. Fresh human monocytes and human macrophages derived from circulating monocytes were used to evaluate both plasticity and anti-inflammatory effects of phytol from HSO at 10-100 mM using FACS analysis, ELISA, and RT-qPCR methods. The quantitative study of the acyclic alcohol fraction isolated from HSO shows that phytol is the most abundant component (167.59 ± 1.81 mg/Kg of HSO). Phytol was able to skew monocyte-macrophage plasticity toward the anti-inflammatory non-classical CD14+CD16++ monocyte phenotype and toward macrophage M2 (CD200Rhigh and MRC-1high), as well as to reduce the production of IL-1β, IL-6, and TNF-α, diminishing the inflammatory competence of mature human macrophages after lipopolysaccharide (LPS) treatment. These findings point out for the first time the reprogramming and anti-inflammatory activity of phytol in human monocyte-macrophages. In addition, our study may help to understand the mechanisms by which phytol from HSO contributes to the constant and progressive plasticity of the human monocyte-macrophage linage.

PMID:35954130 | DOI:10.3390/foods11152366

#CBD #Hemp https://pubmed.ncbi.nlm.nih.gov/35954130/?utm_source=Chrome&utm_medium=rss&utm_campaign=None&utm_content=1jYCQzi_o_qLYr-oQfnMhShgOXkvGma3vcnBGJtrBhuJMOvEVJ&fc=None&ff=20220812162128&v=2.17.7 August 12, 2022 10:00 am

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