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PubMed: Developing Oligo Probes for Chromosomes Identification in Hemp (<em>Cannabis sativa</em> L.)

PubMed: Developing Oligo Probes for Chromosomes Identification in Hemp (<em>Cannabis sativa</em> L.)

Plants (Basel). 2022 Jul 22;11(15):1900. doi: 10.3390/plants11151900.

ABSTRACT

Hemp (Cannabis sativa L., 2n = 20) is a valuable crop that is successfully used as a food, technical and medicinal crop. It is a dioecious plant with an XXXY sex determination system. Some chromosomes of C. sativa have almost the same lengths and centromeric indexes. Cytogenetic markers help to distinguish similar plant chromosomes, including sex chromosomes, which is important for the breeding process. Two repeats (CS-1 and CS-237) were used to develop labeled oligo-probes for rapid and low-cost oligo-FISH. These oligos can be recommended for use as cytological markers to distinguish sex chromosomes (X and Y) and somatic chromosome pairs 3, 6, and 8 by rapid oligo-FISH in a short time.

PMID:35893604 | DOI:10.3390/plants11151900

#CBD #Hemp https://pubmed.ncbi.nlm.nih.gov/35893604/?utm_source=Chrome&utm_medium=rss&utm_campaign=None&utm_content=1jYCQzi_o_qLYr-oQfnMhShgOXkvGma3vcnBGJtrBhuJMOvEVJ&fc=None&ff=20220727162054&v=2.17.7 July 27, 2022 10:00 am

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