PubMed: In vitro metabolism of Tebuconazole, Flurtamone, Fenhexamid, Metalaxyl-M and Spirodiclofen in Cannabis sativa L. (hemp) callus cultures

PubMed: In vitro metabolism of Tebuconazole, Flurtamone, Fenhexamid, Metalaxyl-M and Spirodiclofen in Cannabis sativa L. (hemp) callus cultures

Pest Manag Sci. 2021 Jul 26. doi: 10.1002/ps.6575. Online ahead of print.

ABSTRACT

BACKGROUND: Cannabis sativa L. (C. sativa; hemp) is a medicinal plant producing various cannabinoids. Its consumption is legalized for medical use due to their alleged positive health effects. To satisfy the demand C. sativa plants are grown in contained growth chambers. During indoor propagation pesticides are usually used for an efficient production. However, pesticide registration and safe application in C. sativa has not been investigated in detail.

RESULTS: With this study the metabolic degradation of pesticides in recently established C. sativa callus cultures is examined. Tebuconazole, metalaxyl-M fenhexamid, flurtamone and spirodiclofen were applied at 10 μM for 21 days. Results were compared with metabolism data obtained from Brassica napus L., Glycine max (L.) Merr., Zea mays L. and Tritium aestivum L. callus cultures as well as in metabolism guideline studies. The successfully established C. sativa callus cultures were able to degrade pesticides by oxidation, demethylation, cleavage of ester bonds in phase I as well as glycosylation and conjugation with malonic acid in phase II and III. Initial metabolites were detected after 7 days and were traced during the 21 day.

CONCLUSION: The resulting pathways demonstrate the same main degradation strategies as crop plants. Since metabolites could be the main residue, the exposure of consumers to these residues will be of high importance. We present here an in vitro assay for a first estimation of pesticide metabolism in C. sativa. This article is protected by copyright. All rights reserved.

PMID:34309997 | DOI:10.1002/ps.6575

#CBD #Hemp https://pubmed.ncbi.nlm.nih.gov/34309997/?utm_source=Chrome&utm_medium=rss&utm_campaign=None&utm_content=1jYCQzi_o_qLYr-oQfnMhShgOXkvGma3vcnBGJtrBhuJMOvEVJ&fc=None&ff=20210727070000&v=2.14.5 July 26, 2021 10:00 am

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