Sequential Sampling of Four Hemp Cultivars for Cannabinoids – 2020

Sequential Sampling of Four Hemp Cultivars for Cannabinoids – 2020

Four cultivars of Hemp (Cannabis sativa L.) were planted and grown in a Maury‐Bluegrass siltloam soil at the University of Kentucky North Farm research complex near Lexington, Kentucky. Soil test levels were adequate for P and K. Nitrogen was applied as Urea at 150 lbs. N/A and incorporated prior to planting. The field was treated with 5.5 oz of Spartan Charge and 1 pint of Command per acre for weed control 1 day prior to planting. The cultivars tested in this project were BaOx, Cherry Citrus, Endurance and T1. Female clones of each cultivar were purchased from a licensed hemp grower and transplanted into the field on June 17, 2020.

Cultivars were replicated 4 times in a randomized complete block design. Individual plot size was four rows (42 inches) by 50 feet for a total of 48 plants per plot. Drip irrigation was provided as needed through‐out the early growing season.

Beginning on September 8, 2020 each plot was sampled weekly for THC and CBD analysis. Samples were collected in a manner similar to the compliance sampling protocol used by the Kentucky Department of Agriculture (KDA). The top 20 cm (8 inches) of the uppermost inflorescence was collected from three plants within each plot. Samples were kept separate by plot through analysis. Samples were transported to the KDA where they were dried at 70 degrees C for 24 hours. Dried samples were taken to University of Kentucky Regulatory Services and analyzed for total THC and CBD by GC‐FID. 

The data presented are percent total THC and total CBD on a dry weight basis. The bars presented below are the mean of the four replications and the error bars are the standard deviation of the mean for each cultivar at each sampling time.

[Often the gems are buried in the footnotes. Like this one, in USDA’s 301 page Final Rule. RR]

Leave Your Reply