CannaSearch Daily on CBD + Hemp

Equitable Entry Into the CBD and Marijuana Industry — Vinegar Hill Magazine

Equitable Entry Into the CBD and Marijuana Industry — Vinegar Hill Magazine Yolanda Rush of Otha George Hemp & Wellness, a new Black-owned CBD company said, “It is already a multi-billion dollar industry.” Yolanda's interest … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://www.vinegarhillmagazine.com/news/2021/1/24/e7u5bzh3ljlrnwig4nnycvk8t7op56&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNGwb-1yVCkjm1p9vjcu5hnzh_SQpA January 24, 2021 10:42 am

How Will Global Hemp Derived CBD Oil Market React from 2021 Onwards?

How Will Global Hemp Derived CBD Oil Market React from 2021 Onwards? The report covers the overall growth analysis, industrial strategic policies, market revenue, share, size, and other market growth stimulators. The Hemp … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://murphyshockeylaw.net/energy/1416171/how-will-global-hemp-derived-cbd-oil-market-react-from-2021-onwards/&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNGUGOX4J74EDz4TqOea8uknOHjV5w January 24, 2021 8:48 am

E27 Lamp Retro Hemp Rope Chandelier Ceiling Light Industrial Lamp Home Decor UK – Dronial

E27 Lamp Retro Hemp Rope Chandelier Ceiling Light Industrial Lamp Home Decor UK – Dronial – Creative rope shape in natural hemp color. Light Source: Incandescent light bulb or led bulb (Not Included).. Condition:: New: A brand-new, unused, … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=http://dronial.es/Home-Furniture-%26-DIY-Home-Decor-UK-Lamp-Retro-839745-Ceiling-Lights-%26-Chandeliers/&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNFM-aV3bzb7IHgDVpMFEJ3Q0lPm_g January 24, 2021 5:47 am

50M Dark Green Jute Twine Ball DIY Wrap Gift Hemp Rope Cord String Ball – Dronial

50M Dark Green Jute Twine Ball DIY Wrap Gift Hemp Rope Cord String Ball – Dronial Material: Hemp, Length of Rope: 50 M, Add To Favorites, Lowest Prices Trend frontier Modern Fashion FREE RETURNS & FREE 7-DAY SHIPPING … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=http://dronial.es/Crafts-Gift-Hemp-Rope-Cord-String-Ball-M-123873-Ribbon/&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNGBwcdjVMwuNUm_W9wZSA8oW5yVYQ January 24, 2021 5:41 am

Hemp-based Food Market Innovative Strategy by 2027 | Hemp Oil Canada, Braham & Murray …

Hemp-based Food Market Innovative Strategy by 2027 | Hemp Oil Canada, Braham & Murray … It gives informative data relating to following aspects which are driving the global Hemp-based Food market in terms of the operational and financial … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://murphyshockeylaw.net/uncategorized/1409971/hemp-based-food-market-innovative-strategy-by-2027-hemp-oil-canada-braham-murray-jinzhou-qiaopai-biotech/&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNFJGy1pxsBi5Mz-IHXEbyPXnZECww January 24, 2021 5:26 am

District basketball teams flourishing

District basketball teams flourishing Kate Sears (32) pulls down an offensive rebound, outreaching Asheville's Elly Wright and Watauga teammate Abby Hemp, Jan. 22 at Lentz Eggers … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://www.wataugademocrat.com/sports/district-basketball-teams-flourishing/article_5ef825de-edda-577d-8504-4c38428fa7ac.html&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNGo82dbfZQUIytBbBUb2AP4dNqQjg January 24, 2021 5:15 am

Global Industrial Hemp in Food Market : Industry Analysis and Forecast (2021-2026) – by Type …

Global Industrial Hemp in Food Market : Industry Analysis and Forecast (2021-2026) – by Type … Likewise, it distinguishes a real perspective to help; leading companies focus on key players ( HemPoland, HMI Group, Yunnan Industrial Hemp, … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://nglaustralia.com.au/headlines/78260/global-industrial-hemp-in-food-market-industry-analysis-and-forecast-2021-2026-by-type-application-and-region/&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNE0tJcnmGgZqsQYFrxlWHnRSo5QbA January 24, 2021 5:06 am

Delta to hold hearing on contentious industrial hemp farm

Delta to hold hearing on contentious industrial hemp farm The bylaw is squarely aimed at stopping the outdoor industrial hemp grow operation in the 5700-block of 6th Avenue, a 18-hectare (45-acre) site, … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://www.delta-optimist.com/local-news/delta-to-hold-hearing-on-contentious-industrial-hemp-farm-3276634&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNGy4lyPGMRcnlAh3wMkb243f4e4Xw January 24, 2021 3:33 am

Pakistan women to play Zimbabwe next month

Pakistan women to play Zimbabwe next month Pakistan head coach David Hemp welcomed additional matches for his charges. Article continues after ad. “This is an excellent news for our team as … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://www.dawn.com/news/1603259&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNEkEnk5LWPNOPniTGe83xzg6qQJ4w January 24, 2021 1:41 am

Cannabis Pet Business Launches First Natural Supplements

Cannabis Pet Business Launches First Natural Supplements Developing cannabidiol (CBD) clinically-proven prescription products for pets, that vets can prescribe with confidence, is equally important to Helius … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://www.scoop.co.nz/stories/BU2101/S00125/cannabis-pet-business-launches-first-natural-supplements.htm&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNG4EZTBhR3LPR3fAF-HmrCDpT1wYw January 24, 2021 1:18 am

Fibre made from recycled cloth towels and hemp stronger than cotton – Lindström

Fibre made from recycled cloth towels and hemp stronger than cotton – Lindström Compared to the original hemp knit, the Ioncell knit was more shiny, softer and more durable,' Raiskio tells. The high uniformity and high tenacity of … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://lindstromgroup.com/article/fibre-made-from-recycled-cloth-towels-and-hemp-stronger-than-cotton/&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNGLBgbPiJM015n4-1zK3zoO6vQL4w January 24, 2021 12:46 am

Tag: Industrial Hemp in Chemical

Tag: Industrial Hemp in Chemical Top Key Players Present in Industrial Hemp in Chemical Market Are: Industrial Hemp Manufacturing, Valley Bio, Plains Industrial Hemp Processing, HMI … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://nglaustralia.com.au/tag/industrial-hemp-in-chemical/&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNEHVVIqr4TnFwxCzvy8fkNw1gYXyg January 24, 2021 12:40 am

Tag: Industrial Hemp Manufacturing

Tag: Industrial Hemp Manufacturing Global Market Vision proclaims the addition of new statistical report to it the massive Industrial Hemp in Chemical market for the forecast period of the … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://nglaustralia.com.au/tag/industrial-hemp-manufacturing/&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNETnHgpYa0zvCRTtq1zzPNCgW82FQ January 24, 2021 12:40 am

Tag: Industrial Hemp in Chemical Market Google News

Tag: Industrial Hemp in Chemical Market Google News “Industrial Hemp in ChemicalMarket is growing at a High CAGR during the forecast period 2021-2027. The increasing interest of the individuals in this … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://nglaustralia.com.au/tag/industrial-hemp-in-chemical-market-google-news/&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNGHJxJZaVbEFRbsxcRe7g6cOfNBEA January 24, 2021 12:40 am

Swatch Book Combo (Hemp & Hemp Blends + Exotic Fabrics)

Swatch Book Combo (Hemp & Hemp Blends + Exotic Fabrics) Fabric Swatches of all the available Hemp, Hemp Blend and Hemp Knit Fabrics. Hemp swatch book contains 66 leaflets with 60 types of fabrics in … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://hemis.store/products/swatch-book-combo-hemp-hemp-blends-exotic-fabrics&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNFrtmrsTKYmo_FlvlQtHzNiVhAkrw January 24, 2021 12:38 am

Hemp & CBD Oil Extraction Experts – Experts in Renewables & CBD Extraction

Hemp & CBD Oil Extraction Experts – Experts in Renewables & CBD Extraction FBA has experience inside operational CBD oil extraction facilities. Our engineers utilize modern tools like AutoCad 3D to engineer high quality … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=http://www.frazierbarnes.com/hemp-cbd-oil-extraction&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNF_79fg8C_z10CCFjEgcw7JOvf-Iw January 24, 2021 12:24 am

Louisiana hemp growers affected by USDA's final rule regulating production in the US

Louisiana hemp growers affected by USDA's final rule regulating production in the US “The Louisiana Department of Agriculture and Forestry will work with the legislature during the 2021 legislative session to align Louisiana's hemp law … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://wgno.com/news/louisiana/louisiana-hemp-growers-affected-by-usdas-final-rule-regulating-production-in-the-u-s/&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNEplEKLzsAnUyUAVdwlcRprPUsdTg January 24, 2021 12:00 am

TODAY – Edible Hemp Explained | Facebook

TODAY – Edible Hemp Explained | Facebook It's making its way into milk, wine, chocolate and fine dining. But there are many questions surrounding edible hemp products, despite being legal … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://www.facebook.com/iwakeupwithtoday/videos/322670999120848/&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNHa1l1MxlIGO3bZksmsDyV_JY-dCQ January 23, 2021 11:59 pm

How Much Joyce Meyer CBD Oil Beneficial?

How Much Joyce Meyer CBD Oil Beneficial? Joyce Meyer CBD Oil is made up of hemp oil extract along with cannabinoids that will mix along with the blood vessels present in the body of the … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://patch.com/new-york/midtown-nyc/classifieds/for-sale/198070/how-much-joyce-meyer-cbd-oil-beneficial&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNE_xHJh6qMu2VSFpaaSDtrw2Wb4kQ January 23, 2021 10:41 pm

Cbd distillate liter – Yang Consult

Cbd distillate liter – Yang Consult cbd distillate liter Please contact us to discuss price, payment and shipping … Bulk Full Spectrum Alcohol Extracted 70-80% CBD USA Hemp CBD … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://yangconsult.com/how-to-fgfsk/cbd-distillate-liter.html&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNFPbz6gdOHF8bF3MLvsw6MTfcDpnQ January 23, 2021 10:02 pm

Non-alcohol brands gain from booze ban

Non-alcohol brands gain from booze ban Rethink's range includes oils, capsules and teas made from cannabidiol (CBD), a chemical compound found in the cannabis plant that is gaining … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://www.businesslive.co.za/bt/business-and-economy/2021-01-24-non-alcohol-brands-gain-from-booze-ban/&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNFe2t_rWUN3Ize-IiV1gl7LwGotmA January 23, 2021 9:56 pm

CBD For Dogs? New Research Backs Canine Hemp Oil Use For Epilepsy and Arthritis

CBD For Dogs? New Research Backs Canine Hemp Oil Use For Epilepsy and Arthritis Overall, the results showed that dogs tolerated clinical doses of ABSC Colorado Hemp Oil (CBD) without serious adverse effects in the 6-week long … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://www.einnews.com/pr_news/534971703/cbd-for-dogs-new-research-backs-canine-hemp-oil-use-for-epilepsy-and-arthritis&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNFxM_jApFPYG1yWCeL3Ni9SI2BTeA January 23, 2021 9:22 pm

Queen's Windsor Farm Shop selling 'popular' cannabis-infused drinks

Queen's Windsor Farm Shop selling 'popular' cannabis-infused drinks The farm shop is selling an energy drink called Trip, made from the marijuana compound cannabidiol (CBD). Trip comes in four flavours and has been … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://www.nzherald.co.nz/lifestyle/queens-windsor-farm-shop-selling-popular-cannabis-infused-drinks/765AYBSM3LUSHQIXC6G5RAVTII/&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNHABEi0Edv4yhuHF6X2ha-JK1Ryrw January 23, 2021 9:05 pm

Cannabis-Derived Compounds Cannabichromene and Δ9-Tetrahydrocannabinol Interact and Exhibit Cytotoxic Activity against Urothelial Cell Carcinoma Correlated with Inhibition of Cell Migration and Cytoskeleton Organization.

Cannabis-Derived Compounds Cannabichromene and Δ9-Tetrahydrocannabinol Interact and Exhibit Cytotoxic Activity against Urothelial Cell Carcinoma Correlated with Inhibition of Cell Migration and Cytoskeleton Organization.

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Cannabis-Derived Compounds Cannabichromene and Δ9-Tetrahydrocannabinol Interact and Exhibit Cytotoxic Activity against Urothelial Cell Carcinoma Correlated with Inhibition of Cell Migration and Cytoskeleton Organization.

Molecules. 2021 Jan 17;26(2):

Authors: Anis O, Vinayaka AC, Shalev N, Namdar D, Nadarajan S, Anil SM, Cohen O, Belausov E, Ramon J, Mayzlish Gati E, Koltai H

Abstract
Cannabis sativa contains more than 500 constituents, yet the anticancer properties of the vast majority of cannabis compounds remains unknown. We aimed to identify cannabis compounds and their combinations presenting cytotoxicity against bladder urothelial carcinoma (UC), the most common urinary system cancer. An XTT assay was used to determine cytotoxic activity of C. sativa extracts on T24 and HBT-9 cell lines. Extract chemical content was identified by high-performance liquid chromatography (HPLC). Fluorescence-activated cell sorting (FACS) was used to determine apoptosis and cell cycle, using stained F-actin and nuclei. Scratch and transwell assays were used to determine cell migration and invasion, respectively. Gene expression was determined by quantitative Polymerase chain reaction (PCR). The most active decarboxylated extract fraction (F7) of high-cannabidiol (CBD) C. sativa was found to contain cannabichromene (CBC) and Δ9-tetrahydrocannabinol (THC). Synergistic interaction was demonstrated between CBC + THC whereas cannabinoid receptor (CB) type 1 and type 2 inverse agonists reduced cytotoxic activity. Treatments with CBC + THC or CBD led to cell cycle arrest and cell apoptosis. CBC + THC or CBD treatments inhibited cell migration and affected F-actin integrity. Identification of active plant ingredients (API) from cannabis that induce apoptosis and affect cell migration in UC cell lines forms a basis for pre-clinical trials for UC treatment.

PMID: 33477303 [PubMed – in process]

#CBD #Hemp https://www.ncbi.nlm.nih.gov/pubmed/33477303?dopt=Abstract

Aesculus hippocastanum L. as a Stabilizer in Hemp Seed Oil Nanoemulsions for Potential Biomedical and Food Applications.

Aesculus hippocastanum L. as a Stabilizer in Hemp Seed Oil Nanoemulsions for Potential Biomedical and Food Applications.

Related Articles

Aesculus hippocastanum L. as a Stabilizer in Hemp Seed Oil Nanoemulsions for Potential Biomedical and Food Applications.

Int J Mol Sci. 2021 Jan 17;22(2):

Authors: Jarzębski M, Smułek W, Siejak P, Rezler R, Pawlicz J, Trzeciak T, Jarzębska M, Majchrzak O, Kaczorek E, Kazemian P, Ponieważ-Pawlicz M, Fathordoobady F

Abstract
Nanoemulsion systems receive a significant amount of interest nowadays due to their promising potential in biomedicine and food technology. Using a two-step process, we produced a series of nanoemulsion systems with different concentrations of hemp seed oil (HSO) stabilized with Aesculus hippocastanum L. extract (AHE). Water and commercially-available low-concentrated hyaluronic acid (HA) were used as the liquid phase. Stability tests, including an emulsifying index (EI), and droplet size distribution tests performed by dynamic light scattering (DLS) proved the beneficial impact of AHE on the emulsion’s stability. After 7 days of storage, the EI for the water-based system was found to be around 100%, unlike the HA systems. The highest stability was achieved by an emulsion containing 5% HSO and 2 g/L AHE in water, as well as the HA solution. In order to obtain the detailed characteristics of the emulsions, UV-Vis and FTIR spectra were recorded, and the viscosity of the samples was determined. Finally, a visible microscopic analysis was used for the homogeneity evaluation of the samples, and was compared with the DLS results of the water system emulsion, which showed a desirable stability. The presented results demonstrate the possible use of oil emulsions based on a plant extract rich in saponins, such as AHE. Furthermore, it was found that the anti-inflammatory properties of AHE provide opportunities for the development of new emulsion formulations with health benefits.

PMID: 33477381 [PubMed – in process]

#CBD #Hemp https://www.ncbi.nlm.nih.gov/pubmed/33477381?dopt=Abstract

Assessment of Cannabidiol and Δ9-Tetrahydrocannabiol in Mouse Models of Medulloblastoma and Ependymoma.

Assessment of Cannabidiol and Δ9-Tetrahydrocannabiol in Mouse Models of Medulloblastoma and Ependymoma.

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Assessment of Cannabidiol and Δ9-Tetrahydrocannabiol in Mouse Models of Medulloblastoma and Ependymoma.

Cancers (Basel). 2021 Jan 18;13(2):

Authors: Andradas C, Byrne J, Kuchibhotla M, Ancliffe M, Jones AC, Carline B, Hii H, Truong A, Storer LCD, Ritzmann TA, Grundy RG, Gottardo NG, Endersby R

Abstract
Children with medulloblastoma and ependymoma are treated with a multidisciplinary approach that incorporates surgery, radiotherapy, and chemotherapy; however, overall survival rates for patients with high-risk disease remain unsatisfactory. Data indicate that plant-derived cannabinoids are effective against adult glioblastoma; however, preclinical evidence supporting their use in pediatric brain cancers is lacking. Here we investigated the potential role for Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) in medulloblastoma and ependymoma. Dose-dependent cytotoxicity of medulloblastoma and ependymoma cells was induced by THC and CBD in vitro, and a synergistic reduction in viability was observed when both drugs were combined. Mechanistically, cannabinoids induced cell cycle arrest, in part by the production of reactive oxygen species, autophagy, and apoptosis; however, this did not translate to increased survival in orthotopic transplant models despite being well tolerated. We also tested the combination of cannabinoids with the medulloblastoma drug cyclophosphamide, and despite some in vitro synergism, no survival advantage was observed in vivo. Consequently, clinical benefit from the use of cannabinoids in the treatment of high-grade medulloblastoma and ependymoma is expected to be limited. This study emphasizes the importance of preclinical models in validating therapeutic agent efficacy prior to clinical trials, ensuring that enrolled patients are afforded the most promising therapies available.

PMID: 33477420 [PubMed]

#CBD #Hemp https://www.ncbi.nlm.nih.gov/pubmed/33477420?dopt=Abstract

The Past, Present and Future of Cannabis sativa Tissue Culture.

The Past, Present and Future of Cannabis sativa Tissue Culture.

Related Articles

The Past, Present and Future of Cannabis sativa Tissue Culture.

Plants (Basel). 2021 Jan 19;10(1):

Authors: Monthony AS, Page SR, Hesami M, Jones AMP

Abstract
The recent legalization of Cannabis sativa L. in many regions has revealed a need for effective propagation and biotechnologies for the species. Micropropagation affords researchers and producers methods to rapidly propagate insect-/disease-/virus-free clonal plants and store germplasm and forms the basis for other biotechnologies. Despite this need, research in the area is limited due to the long history of prohibitions and restrictions. Existing literature has multiple limitations: many publications use hemp as a proxy for drug-type Cannabis when it is well established that there is significant genotype specificity; studies using drug-type cultivars are predominantly optimized using a single cultivar; most protocols have not been replicated by independent groups, and some attempts demonstrate a lack of reproducibility across genotypes. Due to culture decline and other problems, the multiplication phase of micropropagation (Stage 2) has not been fully developed in many reports. This review will provide a brief background on the history and botany of Cannabis as well as a comprehensive and critical summary of Cannabis tissue culture. Special attention will be paid to current challenges faced by researchers, the limitations of existing Cannabis micropropagation studies, and recent developments and future directions of Cannabis tissue culture technologies.

PMID: 33478171 [PubMed]

#CBD #Hemp https://www.ncbi.nlm.nih.gov/pubmed/33478171?dopt=Abstract

Aesculus hippocastanum L. as a Stabilizer in Hemp Seed Oil Nanoemulsions for Potential Biomedical and Food Applications.

Aesculus hippocastanum L. as a Stabilizer in Hemp Seed Oil Nanoemulsions for Potential Biomedical and Food Applications.

Related Articles

Aesculus hippocastanum L. as a Stabilizer in Hemp Seed Oil Nanoemulsions for Potential Biomedical and Food Applications.

Int J Mol Sci. 2021 Jan 17;22(2):

Authors: Jarzębski M, Smułek W, Siejak P, Rezler R, Pawlicz J, Trzeciak T, Jarzębska M, Majchrzak O, Kaczorek E, Kazemian P, Ponieważ-Pawlicz M, Fathordoobady F

Abstract
Nanoemulsion systems receive a significant amount of interest nowadays due to their promising potential in biomedicine and food technology. Using a two-step process, we produced a series of nanoemulsion systems with different concentrations of hemp seed oil (HSO) stabilized with Aesculus hippocastanum L. extract (AHE). Water and commercially-available low-concentrated hyaluronic acid (HA) were used as the liquid phase. Stability tests, including an emulsifying index (EI), and droplet size distribution tests performed by dynamic light scattering (DLS) proved the beneficial impact of AHE on the emulsion’s stability. After 7 days of storage, the EI for the water-based system was found to be around 100%, unlike the HA systems. The highest stability was achieved by an emulsion containing 5% HSO and 2 g/L AHE in water, as well as the HA solution. In order to obtain the detailed characteristics of the emulsions, UV-Vis and FTIR spectra were recorded, and the viscosity of the samples was determined. Finally, a visible microscopic analysis was used for the homogeneity evaluation of the samples, and was compared with the DLS results of the water system emulsion, which showed a desirable stability. The presented results demonstrate the possible use of oil emulsions based on a plant extract rich in saponins, such as AHE. Furthermore, it was found that the anti-inflammatory properties of AHE provide opportunities for the development of new emulsion formulations with health benefits.

PMID: 33477381 [PubMed – in process]

#Hemp https://www.ncbi.nlm.nih.gov/pubmed/33477381?dopt=Abstract

The Past, Present and Future of Cannabis sativa Tissue Culture.

The Past, Present and Future of Cannabis sativa Tissue Culture.

Related Articles

The Past, Present and Future of Cannabis sativa Tissue Culture.

Plants (Basel). 2021 Jan 19;10(1):

Authors: Monthony AS, Page SR, Hesami M, Jones AMP

Abstract
The recent legalization of Cannabis sativa L. in many regions has revealed a need for effective propagation and biotechnologies for the species. Micropropagation affords researchers and producers methods to rapidly propagate insect-/disease-/virus-free clonal plants and store germplasm and forms the basis for other biotechnologies. Despite this need, research in the area is limited due to the long history of prohibitions and restrictions. Existing literature has multiple limitations: many publications use hemp as a proxy for drug-type Cannabis when it is well established that there is significant genotype specificity; studies using drug-type cultivars are predominantly optimized using a single cultivar; most protocols have not been replicated by independent groups, and some attempts demonstrate a lack of reproducibility across genotypes. Due to culture decline and other problems, the multiplication phase of micropropagation (Stage 2) has not been fully developed in many reports. This review will provide a brief background on the history and botany of Cannabis as well as a comprehensive and critical summary of Cannabis tissue culture. Special attention will be paid to current challenges faced by researchers, the limitations of existing Cannabis micropropagation studies, and recent developments and future directions of Cannabis tissue culture technologies.

PMID: 33478171 [PubMed]

#Hemp https://www.ncbi.nlm.nih.gov/pubmed/33478171?dopt=Abstract

Free|Trial Original Charlottes Web Cbd Best Cbd Oil For Depression Can Cbd Oil Go Through …

Free|Trial Original Charlottes Web Cbd Best Cbd Oil For Depression Can Cbd Oil Go Through … Original Charlottes Web Cbd Hemp Oil Capsules Walmart Questions About CBD (Cannabidiol) Oil and Hemp Oil Products Original Charlottes Web … #CBD #Hemp https://www.google.com/url?rct=j&sa=t&url=https://melanomaunit.moscow/Cbd/Original-Charlottes-Web-Cbd_D440826.html&ct=ga&cd=CAIyGjJiMjA4MDRjNzhiYTg2MWM6Y29tOmVuOlVT&usg=AFQjCNGSvvAKUQFq7ZVMxYq02aq1SPz24Q January 23, 2021 7:12 pm

Farm, Garden & Outdoors calendar published Jan. 23, 2021

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Doane students and faculty recognized for hemp research

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Mid-infrared spectroscopy as process analytical technology tool for estimation of THC and CBD content in Cannabis flowers and extracts.

Mid-infrared spectroscopy as process analytical technology tool for estimation of THC and CBD content in Cannabis flowers and extracts.

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Mid-infrared spectroscopy as process analytical technology tool for estimation of THC and CBD content in Cannabis flowers and extracts.

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Jan 05;251:119422

Authors: Geskovski N, Stefkov G, Gigopulu O, Stefov S, Huck CW, Makreski P

Abstract
Tetrahydrocannabinol (THC) and cannabidiol (CBD) are the most notable Cannabis components with pharmacological activity and their content in the plant flowers and extracts are considered as critical quality parameters. The new Medical Cannabis industry needs to adopt the quality standards of the pharmaceutical industry, however, the variability of phytocannabinoids content in the plant material often exerts an issue in the inconsistency of the finished product quality parameters. Sampling problems and sample representativeness is a major limitation in the end-point testing, particularly when the expected variation of the product quality parameters is high. Therefore, there is an obvious need for the introduction of Process Analytical Technology (PAT) for continuous monitoring of the critical quality parameters throughout the production processes. Infrared spectroscopy is a promising analytical technique that is consistent with the PAT requirements and its implementation depends on the advances in instrumentation and chemometrics that will facilitate the qualitative and quantitative aspects of the technique. Our present work aims in highlighting the potential of mid-infrared (MIR) spectroscopy as PAT in the quantification of the main phytocannabinoids (THC and CBD), considered as critical quality/material parameters in the production of Cannabis plant and extract. A detailed assignment of the bands related to the molecules of interest (THC, CBD) was performed, the spectral features of the decarboxylation of native flowers were identified, and the specified bands for the acid forms (THCA, CBDA) were assigned and thoroughly explained. Further, multivariate models were constructed for the prediction of both THC and CBD content in extract and flower samples from various origins, and their prediction ability was tested on a separate sample set. Savitskzy-Golay smoothing and the second derivative of the native MIR spectra (1800-400 cm-1 region) resulted in best-fit parameters. The PLS models presented satisfactory R2Y and RMSEP of 0.95 and 3.79% for THC, 0.99 and 1.44% for CBD in the Cannabis extract samples, respectively. Similar statistical indicators were noted for the Partial least-squares (PLS) models for THC and CBD prediction of decarboxylated Cannabis flowers (R2Y and RMSEP were 0.99 and 2.32% for THC, 0.99 and 1.33% for CBD respectively). The VIP plots of all models demonstrated that the THC and CBD distinctive band regions bared the highest importance for predicting the content of the molecules of interest in the respected PLS models. The complexity of the sample (plant tissue or plant extract), the variability of the samples regarding their origin and horticultural maturity, as well as the non-uniformity of the plant material and the flower-ATR crystal contact (in the case of Cannabis flowers) were governing the accuracy descriptors. Taking into account the presented results, ATR-MIR should be considered as a promising PAT tool for THC and CBD content estimation, in terms of critical material and quality parameters for Cannabis flowers and extracts.

PMID: 33477086 [PubMed – as supplied by publisher]

#CBD #Hemp https://www.ncbi.nlm.nih.gov/pubmed/33477086?dopt=Abstract

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Canadian Pot Entrepreneur Escapes Ex-Partners' IP Suit

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The Role of Cannabinoids as Anticancer Agents in Pediatric Oncology.

The Role of Cannabinoids as Anticancer Agents in Pediatric Oncology.

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The Role of Cannabinoids as Anticancer Agents in Pediatric Oncology.

Cancers (Basel). 2021 Jan 05;13(1):

Authors: Andradas C, Truong A, Byrne J, Endersby R

Abstract
Cannabinoids are a group of chemicals that bind to receptors in the human body and, in turn, modulate the endocannabinoid system (ECS). They can be endogenously produced, synthetic, or derived from the plant Cannabis sativa L. Research over the past several decades has shown that the ECS is a cellular communication network essential to maintain multiple biological functions and the homeostasis of the body. Indeed, cannabinoids have been shown to influence a wide variety of biological effects, including memory, pain, reproduction, bone remodeling or immunity, to name a few. Unsurprisingly, given these broad physiological effects, alterations of the ECS have been found in different diseases, including cancer. In recent years, the medical use of cannabis has been approved in different countries for a variety of human conditions. However, the use of these compounds, specifically as anticancer agents, remains controversial. Studies have shown that cannabinoids do have anticancer activity in different tumor types such as breast cancer, melanoma, lymphoma and adult brain cancer. Specifically, phytocannabinoids Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) has been shown to induce apoptosis and inhibit proliferation of adult cancer cells, as well as modulate angiogenesis and metastasis. Despite increasing evidence that cannabinoids elicit antitumor effects in adult cancers, there is minimal data available on their effects in children or in pediatric cancers despite public and clinical demand for information. Here we describe a comprehensive and critical review of what is known about the effects of cannabinoids on pediatric cancers, highlight current gaps in knowledge and identify the critical issues that need addressing before considering these promising but controversial drugs for use in pediatric oncology.

PMID: 33466435 [PubMed]

#CBD #Hemp https://www.ncbi.nlm.nih.gov/pubmed/33466435?dopt=Abstract

Cannabis sativa L. as a Natural Drug Meeting the Criteria of a Multitarget Approach to Treatment.

Cannabis sativa L. as a Natural Drug Meeting the Criteria of a Multitarget Approach to Treatment.

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Cannabis sativa L. as a Natural Drug Meeting the Criteria of a Multitarget Approach to Treatment.

Int J Mol Sci. 2021 Jan 14;22(2):

Authors: Stasiłowicz A, Tomala A, Podolak I, Cielecka-Piontek J

Abstract
Cannabis sativa L. turned out to be a valuable source of chemical compounds of various structures, showing pharmacological activity. The most important groups of compounds include phytocannabinoids and terpenes. The pharmacological activity of Cannabis (in epilepsy, sclerosis multiplex (SM), vomiting and nausea, pain, appetite loss, inflammatory bowel diseases (IBDs), Parkinson’s disease, Tourette’s syndrome, schizophrenia, glaucoma, and coronavirus disease 2019 (COVID-19)), which has been proven so far, results from the affinity of these compounds predominantly for the receptors of the endocannabinoid system (the cannabinoid receptor type 1 (CB1), type two (CB2), and the G protein-coupled receptor 55 (GPR55)) but, also, for peroxisome proliferator-activated receptor (PPAR), glycine receptors, serotonin receptors (5-HT), transient receptor potential channels (TRP), and GPR, opioid receptors. The synergism of action of phytochemicals present in Cannabis sp. raw material is also expressed in their increased bioavailability and penetration through the blood-brain barrier. This review provides an overview of phytochemistry and pharmacology of compounds present in Cannabis extracts in the context of the current knowledge about their synergistic actions and the implications of clinical use in the treatment of selected diseases.

PMID: 33466734 [PubMed – in process]

#CBD #Hemp https://www.ncbi.nlm.nih.gov/pubmed/33466734?dopt=Abstract

The antimicrobial potential of cannabidiol.

The antimicrobial potential of cannabidiol.

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The antimicrobial potential of cannabidiol.

Commun Biol. 2021 Jan 19;4(1):7

Authors: Blaskovich MAT, Kavanagh AM, Elliott AG, Zhang B, Ramu S, Amado M, Lowe GJ, Hinton AO, Pham DMT, Zuegg J, Beare N, Quach D, Sharp MD, Pogliano J, Rogers AP, Lyras D, Tan L, West NP, Crawford DW, Peterson ML, Callahan M, Thurn M

Abstract
Antimicrobial resistance threatens the viability of modern medicine, which is largely dependent on the successful prevention and treatment of bacterial infections. Unfortunately, there are few new therapeutics in the clinical pipeline, particularly for Gram-negative bacteria. We now present a detailed evaluation of the antimicrobial activity of cannabidiol, the main non-psychoactive component of cannabis. We confirm previous reports of Gram-positive activity and expand the breadth of pathogens tested, including highly resistant Staphylococcus aureus, Streptococcus pneumoniae, and Clostridioides difficile. Our results demonstrate that cannabidiol has excellent activity against biofilms, little propensity to induce resistance, and topical in vivo efficacy. Multiple mode-of-action studies point to membrane disruption as cannabidiol’s primary mechanism. More importantly, we now report for the first time that cannabidiol can selectively kill a subset of Gram-negative bacteria that includes the ‘urgent threat’ pathogen Neisseria gonorrhoeae. Structure-activity relationship studies demonstrate the potential to advance cannabidiol analogs as a much-needed new class of antibiotics.

PMID: 33469147 [PubMed – in process]

#CBD #Hemp https://www.ncbi.nlm.nih.gov/pubmed/33469147?dopt=Abstract

Association between Friends' Use of Nicotine and Cannabis and Intake of both Substances among Adolescents.

Association between Friends' Use of Nicotine and Cannabis and Intake of both Substances among Adolescents.

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Association between Friends’ Use of Nicotine and Cannabis and Intake of both Substances among Adolescents.

Int J Environ Res Public Health. 2021 Jan 15;18(2):

Authors: Herold R, Boykan R, Eliscu A, Alcalá HE, Goniewicz ML

Abstract
Nicotine and cannabis use are common among adolescents and may be associated with behavioral problems, poor academic outcomes and use disorders. The goals of this analysis were the following: (1) Describe the influence of friends’ nicotine and cannabis smoking and vaping on self-reported use. (2) Describe the relationship between friends’ nicotine and cannabis use on participants’ urinary biomarkers of nicotine (cotinine) and cannabis (11-nor-9-carboxy-Δ⁸tetrahydrocannabinol=THC-COOH). This is a secondary analysis of survey and biomarker data collected in adolescents aged 12-21 between April 2017 and April 2018, in Long Island, New York. Bivariate and multivariable analyses were conducted using SPSS 26. A cutoff value of ≥10 ng/mL was used to signify recent usage for urinary cotinine and THC-COOH levels. Over one-third of the 517 surveyed adolescents reported using tobacco and one-third reported using cannabis. A significant relationship between friends’ substance use and self-use was found. For both tobacco and cannabis, over 90% (p < 0.01) of participants with urinary biomarker levels above cutoff had friends who used the respective substance. Friends' nicotine and friends' cannabis use were each independently associated with urinary biomarker levels for those substances (for nicotine, beta = 88.29, p = 0.03; for cannabis, beta = 163.58, p = 0.03). Friends' use of nicotine and cannabis is associated with adolescents' intake, as well as the physiological exposure to those substances. These findings underscore the importance of including peer influence in the discussion with adolescents about tobacco and cannabis use.

PMID: 33467405 [PubMed – in process]

#CBD #Hemp https://www.ncbi.nlm.nih.gov/pubmed/33467405?dopt=Abstract